To this date, no efficient treatment without side effects or risk of recurrence is available. The lifetime risk for undergoing POP surgery is estimated to 11.1–19% with a risk of further reoperation up to 30% 22. This disorder can lead to pelvic organ prolapse (POP) seen as a herniation of the bladder, uterus or intestines into the vagina 21. Half of the female population over the age of 50 experiences disorders of the pelvic floor caused by weakening and rupture of supportive connective tissue sheet or fascia 20. 19 both demonstrated CTGF loaded 3D scaffold was capable of fostering fibroblastic differentiation in vitro. Our previous study 18 and the study reported by Tong et al. Until recently, connective tissue growth factor (CTGF) or CCN2, a member of CCN family 15 has been identified to sufficiently direct MSCs differentiation towards fibroblasts 16 and promote connective tissue healing in a rodent injury model 17. In association with TGFβ1, insulin-like growth factor-I (IGF-I) and bone morphogenic protein 2 (BMP-2) were known to induce the differentiation of MSCs into chondrocytes 14. The differentiation of MSCs into mesenchymal lineage is known to be controlled by diverse transcription factors and signaling cascades such as Hedgehog 11, NEL-like protein 1 (NELL-1) 12 and β catenin-dependent Wnt 13. Previous studies have shown FGF-2 or basic fibroblast growth factor (b-FGF) to be able to increase rat, rabbit, canine and human MSCs proliferation and maintains their multilineage differentiation potential during in vitro expansion 7, 8, 9, 10.Īpart from the expansion, the prospect of controlling MSC differentiation is a crucial regulatory and clinical requirement. Fibroblast growth factors (FGFs) are a family of growth factors involved in many functions such as cell proliferation, migration, and differentiation and are critically important in tissue development, maintenance and wound repair 6. Some cytokines have been identified to enhance the proliferation of MSCs, such as Platelet-derived growth factor (PDGF) through the activation of c-Jun N-terminal kinase (JNK) signaling 4, Transforming growth factor β1 (TGFβ1) inducing the rapid nuclear translocation of β-catenin in a Smad3-dependent manner 5. However, MSCs survival and incorporation at the graft are still crucial issues for transplantations. These characteristics make them an attractive candidate for biological cell-based tissue repair approaches 3. It has also been suggested that they can modulate the host immune responses when transplanted 2. This solution can then be diluted into other aqueous buffers.Mesenchymal stem cells (MSCs) can be efficiently isolated from adult bone marrow, are capable of extended proliferation and hold the potential to differentiate into mesenchymal lineages including osteoblasts, chondrocytes, and adipocytes 1. Reconstitute in 5 mM Tris, pH 7.6 to a concentration of 0.1-1.0 mg/mL. Lyophilized from a 1 mg/ml solution of 5 mM Tris pH 7.6 plus 150 mM NaCl.Ĭentrifuge the vial prior to opening. It is also referred to as BFGF (basic fibroblast growth factor). The recombinant human FGF-2 is a 17.2kDa protein containing 154 amino acid residues. FGF-2 is a basic heparin binding growth factor. The gene FGF2 (fibroblast growth factor 2) is mapped to human chromosome 4q28. The FGF2 mRNA is a target of miRNA miR-503. High levels of FGF2 are present in the kidney of children with HIV (human immunodeficiency virus)-renal diseases. FGF2 is known to stimulate angiogenesis and is associated with cancer development. It plays an important role in spinal cord injury repair. FGF2 also determines the fate of neural precursor cells and promotes neuronal axon regeneration. It stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. in growing embryonic neural stem/progenitor cells (NSPCs) in a 2D cultureįGF2 (fibroblast growth factor 2)-human has been used in endothelial colony assay.įGF2 (fibroblast growth factor 2, also referred to as BFGF (basic fibroblast growth factor)) interacts with FGFR1 (fibroblast growth factor receptor 1), FGFR2 and FGFR3.as a component in the neurobasal growth medium.as medium supplement for E9.5 neuroepithelial cell culture.
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